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Make the 250 your Main Drive, use the 400 for storage.
If you put the 2 250's in at the same time, and use norton ghost from the old primary, to the new, then take the old one out and connect it to the 2nd channel, put the new one in its place on the first channel. Boot off that, and format the old disk. Then connect your 400 to the 2nd channel. Then you'll have what you need.
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